|Position||Host institution||PhD enrolment||Start date||Duration|
|ESR1||P1-USAAR||Y (at USAAR)||early 2017||36 months|
Role of glial transmitter receptors in epileptic network function in vivo
The aim of this project is to provide information about communication mechanisms between neuronal and glial cell populations in an animal model of temporal lobe epilepsy. Glial network activities will be assessed primarily as Ca2+ signals since these have integrative functions. Electrical activity such as extracellular field activity in slice preparations or EEG recordings from experimental animals will be used for complementation and confirmation.
Electrophysiology of acute brain slices and in vivo imaging of Ca2+ signals using two-photon laser-scanning microscopy (2P-LSM) will be employed as well as telemetric EEG recordings and behavioural analysis. Molecular and immunohistochemical techniques will be applied to determine the extent of receptor knockout and subsequent changes in the adjacent neighbourhood.