|Position||Host institution||PhD enrolment||Start date||Duration|
|ESR2||P1-USAAR||Y (at USAAR)||early 2017||36 months|
Molecular and cellular mechanisms of pathological network function in mouse models of absence epilepsy
To evaluate the role of distinct glial transmitter receptors in the generation and duration of absence seizures in respective animal models.
Knockout mice with inducible gene deletion will be investigated by in vivo 2P-LSM using intracellular Ca2+ as indicator of glial network activity. After induced gene ablation, chemical seizures will be evoked. Ca2+ signals as well as EEGs will be recorded in the affected mice. In addition, molecular and immunohistochemical techniques will be applied to determine and quantify the extent of receptor knockout and subsequent changes in the adjacent neighbourhood.